Direct immunoperoxidase method for demonstrating Chlamydia psittaci in tissue sections.

Direct immunoperoxidase method for demonstrating Chlamydia psittaci in tissue sections.

A rapid method for staining inclusions of Chlamydia psittaci and Chlamydia trachomatis.

A new staining method was developed for the detection of inclusions of Chlamydia psittaci and Chlamydia trachomatis inclusions in cell cultures. Using a combination of methyl green and neutral red stains and washing at pH 5.0, inclusions were stained red while cell cytoplasm was pale pink and cell nuclei were pale green. The method was significantly better than Giemsa staining and comparable to...

Immunoperoxidase staining for identification of Aspergillus species in routinely processed tissue sections.

AIMS To evaluate the performance of an immunoperoxidase stain using the monoclonal antibody EB-A1 to detect Aspergillus species in formalin fixed, paraffin wax embedded tissue. METHODS The monoclonal antibody EB-A1 directed against galactomannan was used to detect Aspergillus species in 23 patients with suspected or confirmed invasive aspergillosis. Immunostaining was performed on formalin fi...

Chlamydia psittaci in birds of prey, Sweden

BACKGROUND Chlamydia psittaci is an intracellular bacterium primarily causing respiratory diseases in birds but may also be transmitted to other animals, including humans. The prevalence of the pathogen in wild birds in Sweden is largely unknown. METHODS DNA was extracted from cloacae swabs and screened for C. psittaci by using a 23S rRNA gene PCR assay. Partial 16S rRNA and ompA gene fragmen...

The Use of indirect Immunoperoxidase Assay in Diagnosis of Type A (H9N2) Avian Influenza Virus Antigen on Frozen Tissue Sections